Compositions and methods for in vitro sorting of molecular and cellular libraries in water-in-oil-in-water emulsions.

Citation:

Dan Tawfik, Bernath, Kalia , Aharoni, Amir , Peisajovich, Sergio , Griffiths, Andrew D, Mastrobattista, Enrico , and Magdassi, Shlomo. . 2007. “Compositions And Methods For In Vitro Sorting Of Molecular And Cellular Libraries In Water-In-Oil-In-Water Emulsions.”.

Abstract:

The present invention provides an in vitro system for compartmentalization of mol. or cellular libraries and provides methods for selection and isolation of desired mols. or cells from the libraries. The library includes a plurality of distinct mols. or cells encapsulated within a water-in-oil-in-water emulsion. The emulsion includes a continuous external aq. phase and a discontinuous dispersion of water-in-oil droplets. The internal aq. phase of a plurality of such droplets comprises a specific mol. or cell that is within the plurality of distinct mols. or cells of the library. Thus, for example, mutants with improved β-galactosidase activity can be selected from a random mutagenesis library of evolved β-galactosidase lacZ gene using a double emulsion selection system. [on SciFinder(R)]

Notes:

CAPLUS AN 2007:383953(Patent)